Leica LMD7000- laser microdissection microscope

User Manual

Features

  • Applications include: single cell isolation for extraction of DNA, RNA, protein or metabolites from nearly any biological system, allowing downstream (cell-specific) analyses such as for example PCR, sequencing, qRT-PCR, microarray, genetic fingerprinting, 2-D protein gels, mass-spec analyses (LC-MS/MS / MALDI). See Leica science lab for more information.
  • Fully automated high-end upright microscope (DM6000B). Dedicated LMD objectives available: 5x, 10x, 20x, 40x and 63x.
  • Special membrane coated (PEN) glass or frame slides is recommended for mounting tissue sections. Three-slide holder with rapid slide scans.
  • Fully integrated fluorescence, allows live cutting within bright-field and fluorescence (BGR filters for DAPI, FITC, TRITC/PI) mode.
  • UV laser 349 nm solid state diode (120 µJ, pulse frequency 10 – 5000 Hz, pulse length < 4 ns), with continuously adjustable laser aperture, speed and focus.
  • Gravity collection into standard PCR microfuge cals (0.2, 0.5ml), 8-strip wells, or  Petri dishes with or without special membrane (or Leica ibidi slides).
  • Annotate then cut or cut in real-time cutting with a Pen-screen.

Publications

  • Please acknowledge funding for this instrument as follows: “This work utilized a LEICA 7000 laser microdissection microscope purchased with funding from a National Institutes of Health SIG grant 1S10OD016350-01.”
  • Please contact Dr. Martha Campbell-Thompson with any questions regarding this instrument or appropriate citation.

Current publications

  • Han S, et al. The pancreatic tumor microenvironment drives changes in miRNA expression that promote cytokine production and inhibit migration by the tumor associated stroma. Oncotarget, 2016. pdf

Fees and Invoicing

Please visit the Fee page for information on costs and billing.

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