Tissue Microarray

 

The major goal of this facility is to provide tissue microarray (TMA) capabilities from processing samples into donor blocks and evaluating regions of interest to TMA design, construction, sectioning, and staining. Any stain that can be used on a paraffin section can be performed on a TMA slide. Fees for TMA construction are based on tech support time and supplies. Individuals can also construct their own TMA through self-service (Arraymold YouTUBE).and72arraymolds

Benefits

Arrays are 1. Cost effective- view or stain many samples on one slide instead of many individual slides, 2.Optimizing antibodies or probes- optimize an antibody or ISH probe across many different organs or species on one slide instead of staining multiple slides, 3. Consistency- all samples are processed in batch thus differences between samples due to sample rather than technical issues

Methods

Using a H&E stained slide of the donor block for guidance, the area(s) of interest is removed as a core from the donor block with the appropriate sized needle (Beechler) or punch (Arraymold) then transferred into the recipient block. The block is resealed with paraffin and stored at -20 until sectioned. TMA slides are also sealed with paraffin to maximize antigen stability before staining.

Tissue microarrayer

Beechler TMA Configurations
Needle size
Spacing between samples
Array Format
Total # Cores
0.6 mm
0.2 mm
20 x 20
400
1.0 mm
0.3 mm
16 x 13
208
1.5 mm
0.4 mm
11 x 9
99