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The Olympus VS200 slide scanner was obtained through an NIH Shared Instrumentation Grant and has been installed in D11-35. Assisted scanning will be performed by MPC staff and charged on a per-slide cost basis. Unassisted use can be performed following training. The VS200 produces fast scans at 20x or 40x…
Need to run your Nanostring nCounter assay for multiplex RNA, DNA or protein targets? The Molecular Pathology Core maintains a nCounter FLEX instrument and provides technical support to run the instrument. Contact Ann Fu through the MPC mailbox (molecular@ufl.edu) for initial free consultations or information about this instrument. For information…
MPC has joined the BioImaging North American (BINA) consortium. BioImaging North America (BINA) was started during the 2018 “Frontiers in Microscopy Technologies and Strategies for Bioimaging Centers Network” meeting on Janelia Research Campus. It was registered as a US non-profit organization in June 2018 and until the Spring of 2021 was…
In August, we bid a fond farewell to Xian (Lily) Li, postdoctoral fellow, in the Core for two years, who returned to China. She worked tirelessly to provide histology and immunohistology services to our users, particularly during the 2020 pandemic. We hope she can return to the Core in a…
We are pleased to offer the Nanostring GeoMX platform through the Molecular Pathology Core and Department of Surgery’s Laboratory of Inflammation Biology and Surgical Science. Technical support is required with the user providing reagents and samples. Please contact us before planning as there are specific requirements for slide preparations. More…
Dr. Cheng, Assistant Professor, is a cancer and nutritional epidemiologist who joined UF in 2016. His studies have two main elements: 1) studying the role of genetic susceptibility, environmental exposure, and their interactions; and 2) investigating molecular markers in cancer subtypes to elucidate cancer etiologies. Current efforts emphasize molecular pathological…
A recent publication by University of Florida investigators highlights the utility of laser microdissection to separate pancreatic ductal epithelium and stroma. Dr. Song Han and colleagues published their studies in Oncotarget describing alterations in miRNA important in pancreatic tumor biology. Laser microdissection was performed by Dr. David Gonzalo, Chief Gastrointestinal…
Dr. Campbell-Thompson recently hosted Dr. Edward Stack from PerkinElmer who gave a seminar titled “Phenoptics: Navigating the immunological microenvironment, in situ, to uncover the hidden clues of cancer immunology.” Use of multispectral staining and image analysis was demonstrated for a variety of tumor types with immune cell phenotyping for personalized…
Dr. Ann Fu performed in situ hybridization for LINC00473 using an ACD RNAScope customized probe with Dr. Lizi Wu (cAMP/CREB-regulated LINC00473 marks LKB1-inactivated lung cancer and mediates tumor growth, JCI 2016). Enhanced LINC00473 expression was highly correlated with LKB1 mutational status and was associated with poor survival in human lung…
Dr. Campbell-Thompson and colleagues recently published an article on the immunophenoytyping of islet inflammation and residual beta cell mass in organ donors with type 1 diabetes in the March, 2016 issue of Diabetes. One of their images was selected for the journal’s cover. The multiple immunofluorescence staining was done by…
