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IHC

Welcome Department of Medicine EM Core Users

The UF Department of Medicine Electron Microscopy core will be closing Jan. 31, 2021 with the retirement of Dr. Jill Verlander, Director. Mr. Chao Chen will be joining the MPC staff and continue providing light microscopy services used in the EM Core, including polyester wax embedding, sectioning and staining (see…

MPC Reopening with Modified Hours – May 12, 2020

Molecular Pathology Core staff members are pleased to reopen the facility with limited hours from 10 am- 3 pm for sample submissions and pickup in Dental Tower D11-41. Technical support remains available during regular hours (8:30 am to 4:30 pm) to help you with your research needs and answer any…

New: Precisionary Instruments Compresstome VF-300-OZ

A Compresstome VF-300-oz is now available in the Molecular Pathology Core for assisted or unassisted use. This vibrating microtome is capable of sectioning both fixed and live tissues with thicknesses ranging from 3 µm to 2 mm. Sterile conditions can be used for living slice studies suitable for electrophysiology…

Ki67 IHC Human Tumor

In this immunohistochemistry (IHC) single stain, Ki67+ nuclei are shown in brown chromogen (DAB) in a human tumor indicating a high proliferation rate. All nuclei are counterstained with hematoxylin. Ki67 IHC is also available for mouse and rat tissues and in combination with other primary antibodies.  …

Quadruple IHC Stain

Quadruple or multiplex IHC is becoming more mainstream. Shown are islets from human, rat, and pig stained for somatostatin (SS, green), glucagon (red), insulin (blue) and pancreatic polypeptide (black). This staining was done using chromogen kits given to the MPC by Biocare Medical. Human: Rat: Pig:  …

New Customer Feedback Form

We, the Molecular Pathology Core, have recently added a Customer Feedback Form to our website. We want to hear back from our submitters. We encourage you to fill out a feedback form to let us know what you think of our services.  …

Multiplex staining: PE Opal

Dr. Campbell-Thompson recently hosted Dr. Edward Stack from PerkinElmer who gave a seminar titled “Phenoptics: Navigating the immunological microenvironment, in situ, to uncover the hidden clues of cancer immunology.”  Use of multispectral staining and image analysis was demonstrated for a variety of tumor types with immune cell phenotyping for personalized…

RNA in situ hybridization: Journal Clinical Investigation, June 2016

Dr. Ann Fu performed in situ hybridization for LINC00473 using an ACD RNAScope customized probe with Dr. Lizi Wu (cAMP/CREB-regulated LINC00473 marks LKB1-inactivated lung cancer and mediates tumor growth, JCI 2016).  Enhanced LINC00473 expression was highly correlated with LKB1 mutational status and was associated with poor survival in human lung…

Diabetes March 2016 Cover Image

Dr. Campbell-Thompson and colleagues recently published an article on the immunophenoytyping of islet inflammation and residual beta cell mass in organ donors with type 1 diabetes in the March, 2016 issue of Diabetes. One of their images was selected for the  journal’s cover. The multiple immunofluorescence staining was…

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