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IHC

MPC Staff Contribute to Biomaterials Advances Publication on Aptamer-Functionalized Hydrogels

We are pleased to highlight a recent publication by Dr. Nikolaos Pipis in Biomaterials Advances (Elsevier), titled “Injectable aptamer-functionalized nucleic acid–collagen hydrogels as a bioactive platform for angiogenic applications.” This work represents the culmination of Dr. Pipis’s doctoral research at the University of Florida and presents the first in vivo…

Quadruple IHC Stain

Quadruple or multiplex IHC is becoming more mainstream. Shown below are islets from three species (human, rat, and pig) stained for somatostatin (SS, green), glucagon (GCG, red), insulin (Ins, blue) and pancreatic polypeptide (PP, black). This staining was done using OPAL chromogen kits given to the MPC by Biocare Medical.

New Customer Feedback Form

We, the Molecular Pathology Core, have recently added a Customer Feedback Form to our website. We want to hear back from our submitters. We encourage you to fill out a feedback form to let us know what you think of our services.  …

Multiplex staining: PE Opal

Dr. Campbell-Thompson recently hosted Dr. Edward Stack from PerkinElmer who gave a seminar titled “Phenoptics: Navigating the immunological microenvironment, in situ, to uncover the hidden clues of cancer immunology.”  Use of multispectral staining and image analysis was demonstrated for a variety of tumor types with immune cell phenotyping for personalized…

RNA in situ hybridization: Journal Clinical Investigation, June 2016

Dr. Ann Fu performed in situ hybridization for LINC00473 using an ACD RNAScope customized probe with Dr. Lizi Wu (cAMP/CREB-regulated LINC00473 marks LKB1-inactivated lung cancer and mediates tumor growth, JCI 2016).  Enhanced LINC00473 expression was highly correlated with LKB1 mutational status and was associated with poor survival in human lung…

Diabetes March 2016 Cover Image

Dr. Campbell-Thompson and colleagues recently published an article on the immunophenoytyping of islet inflammation and residual beta cell mass in organ donors with type 1 diabetes in the March, 2016 issue of Diabetes. One of their images was selected for the  journal’s cover. The multiple immunofluorescence staining was done by…