Mouse Aorta Stained With H&E
Our users often request special services for sectioning or staining. On this particular project, mouse aorta is shown by H&E stain. (Mouse aorta stained with hematoxylin and eosin) …
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Quadruple IHC Stain
Quadruple or multiplex IHC is becoming more mainstream. Shown below are islets from three species (human, rat, and pig) stained for somatostatin (SS, green), glucagon (GCG, red), insulin (Ins, blue) and pancreatic polypeptide (PP, black). This staining was done using OPAL chromogen kits given to the MPC by Biocare Medical.
New Customer Feedback Form
We, the Molecular Pathology Core, have recently added a Customer Feedback Form to our website. We want to hear back from our submitters. We encourage you to fill out a feedback form to let us know what you think of our services. …
Laser microdissection for pancreatic cancer- Song Han et al, July 2016
A recent publication by University of Florida investigators highlights the utility of laser microdissection to separate pancreatic ductal epithelium and stroma. Dr. Song Han and colleagues published their studies in Oncotarget describing alterations in miRNA important in pancreatic tumor biology. Laser microdissection was performed by Dr. David Gonzalo, Chief Gastrointestinal…
Multiplex staining: PE Opal
Dr. Campbell-Thompson recently hosted Dr. Edward Stack from PerkinElmer who gave a seminar titled “Phenoptics: Navigating the immunological microenvironment, in situ, to uncover the hidden clues of cancer immunology.” Use of multispectral staining and image analysis was demonstrated for a variety of tumor types with immune cell phenotyping for personalized…
RNA in situ hybridization: Journal Clinical Investigation, June 2016
Dr. Ann Fu performed in situ hybridization for LINC00473 using an ACD RNAScope customized probe with Dr. Lizi Wu (cAMP/CREB-regulated LINC00473 marks LKB1-inactivated lung cancer and mediates tumor growth, JCI 2016). Enhanced LINC00473 expression was highly correlated with LKB1 mutational status and was associated with poor survival in human lung…
Diabetes March 2016 Cover Image
Dr. Campbell-Thompson and colleagues recently published an article on the immunophenoytyping of islet inflammation and residual beta cell mass in organ donors with type 1 diabetes in the March, 2016 issue of Diabetes. One of their images was selected for the journal’s cover. The multiple immunofluorescence staining was done by…
Happy Holidays 2016 from all of us
Happy holidays to everyone. The Molecular Pathology Core will be closed Dec. 24 through the UF break and reopen Monday Jan. 4. The last day to submit fixed samples for paraffin processing will be Monday, Dec. 21.
Laser Capture Microdissection- brightfield or fluorescence
The Leica 7000 is available for bright-field and fluorescence sample microdissection of human, animal or plant materials. Paraffin or fresh frozen samples are placed on PEN membrane slides. Please contact Dr. Ann Fu to discuss your project needs and arrange use. Total RNA amount and integrity isolated from any sample…
Laser Capture Microdissection- Leica 7000
The Leica 7000 is now available for brightfield and fluorescence sample microdissection. Paraffin and frozen samples can be used with nuclei acid isolation. Numerous options are available including live cell capture. Please contact Dr. Ann Fu to discuss your project needs and arrange use.