Leica LMD7000- laser microdissection microscope

User ManualLaser Microdissection

Features

  • Applications include single cell isolation for extraction of DNA, RNA, protein or metabolites from nearly any biological system, allowing downstream (cell-specific) analyses such as for example PCR, sequencing, qRT-PCR, microarray, genetic fingerprinting, 2-D protein gels, mass-spec analyses (LC-MS/MS / MALDI). See Leica science lab for more information.
  • Fully automated high-end upright microscope (DM6000B). Dedicated LMD objectives available: 5x, 10x, 20x, 40x and 63x.
  • Special membrane coated (PEN) glass or frame slides is recommended for mounting tissue sections. Three-slide holder with rapid slide scans.
  • Fully integrated fluorescence allows live cutting within bright-field and fluorescence (BGR filters for DAPI, FITC, TRITC/PI) mode.
  • UV laser 349 nm solid state diode (120 µJ, pulse frequency 10 – 5000 Hz, pulse length < 4 ns), with continuously adjustable laser aperture, speed, and focus.
  • Gravity collection into standard PCR microfuge caps (0.2, 0.5ml), 8-strip wells, or  Petri dishes with or without special membrane (or Leica ibidi slides).
  • Annotate then cut or cut in real-time cutting with a Pen-screen.

Publications

  • Please acknowledge funding for this instrument as follows: “This work utilized a LEICA 7000 laser microdissection microscope purchased with an NIH shared instrumentation grant 1S10OD016350-01.”
  • Please contact Dr. Martha Campbell-Thompson with any questions regarding this instrument or appropriate citation.

Current publications

  • Dou M, et. al. Nanowell-mediated two-dimensional liquid chromatography enables deep proteome profiling of <1000 mammalian cells. Chemical Science. 2018.
  • Zhu Y, et. al. Nanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells. Nat Commun. 2018.
  • Han S, et al. The pancreatic tumor microenvironment drives changes in miRNA expression that promote cytokine production and inhibit migration by the tumor-associated stroma. Oncotarget, 2016. link

Fees and Invoicing

Please visit the Fee page for information on costs and billing.

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