- Initial training is required before you use any instrument for the first time. Users are responsible for their consumables such as low profile microtome blades (cryostat), slides, and slide boxes. Blades, slides, and boxes can be purchased from the MPC.
- Core staff maintain the equipment, offer suggestions on protocols, and perform initial training sessions.
- Equipment rentals are rounded up to the nearest half-hour, eg. 30 minutes minimum.
- Microscopes including slide scanner- clean up the coverslip of your slides with a Kimwipe or cotton-tips moistened with 70% ethanol or Windex. Clean microscope objectives only with provided lens paper.
- If you publish work using one of our instruments, we appreciate your acknowledgement of the core service with a statement like “This work has been supported in part by the Molecular Pathology Core in the Department of Pathology, Immunology and Laboratory Medicine at University of Florida College of Medicine.
- Minimum time on reserved instruments is in 30 minute increments to allow time for set-up and clean-up.
- Please make sure to write down the log-in and out time on the log book. It also helps to track the usage of instrument components.
- The maximum time for a session a day is 4 hour, with exception for specific conditions.
- Paraffin embedding station – Histocentre in D11-41
- Cryostat – H550 in D11-33
- Microtomes – Leica and Microm
- Compresstome vibratome VF-300-OZ in D11-39- vibrating microtome for cutting tissue slices, similar to cryostats and vibrating microtomes. The VF-300 sections is fully automated for sectioning fixed and live tissues, with down to 1-µm precision. This microtome can cut tissue slice thicknesses ranging from 3-µm to 2000-µm, at 1-µm intervals.
- Leica 7000 Laser Microdissection Microscope (LDM)
- Zeiss Axioskop 2 Plus Fluorescence with dual Axiocam hr cameras (color and monochrome)in D11-35A
- Image analysis computer workstation in D11-37