- Initial training or orientation by Core staff is required for all instruments.
- Users are responsible for supplying consumables, including blades, slides, and slide boxes.
- Fill in Equipment use logs with start and end times and calculate the total time (30-minute intervals).
- Failure to use properly and clean up afterward may lead to loss of equipment use.
- All users are responsible for equipment damage incurred through their use.
- Failure to note another person’s damage to an instrument does not absolve one from responsibility discovered later by MPC personnel.
Reservations
- Equipment Reservations Calendar
- Equipment rentals are rounded up to the nearest half-hour, eg. 30 minutes minimum.
- Regular ‘Working Hours Access’ is from 8:30 am to 4:30 pm, Monday through Friday. Closed UF Holidays.
- ‘After-Hours Access’ is defined as hours outside of regular hours. Certain users can have after-hours access based on demonstrated competency of use.
- Limited rental time is available for equipment also needed for fee-for-service submissions.
- Calendar/Add/Edit: Pick the date, add your name and PI, and choose equipment under Category dropdowns. Create an Event to save.
- You will receive an email reminder of your reservation.
Equipment
- Paraffin embedding station – D11-41
- Cryostat- D11-33
- Cytospin- D11-33
- Microtome- D11-33
- Compresstome vibratome VF-300-OZ in D11-41A- vibrating microtome for cutting tissue slices, similar to cryostats and vibrating microtomes. The VF-300 sections is fully automated for sectioning fixed and live tissues, with down to 1-µm precision. This microtome can cut tissue slice thicknesses ranging from 3-µm to 2000-µm, at 1-µm intervals.
- Free use of multi-headed Olympus brightfield upright microscope in D11-35.
- Leica 7000 Laser Microdissection Microscope (LMD)
- Zeiss Axioskop 2 Plus Fluorescence with LED illuminator and both Axiocam hr cameras (color and monochrome) in D11-35A
- 2.5x, 10x, 20x, 40x, 63x (oil)
- Fluorescence Filters:
- Zeiss #01- DAPI, AF350/405, AMCA
- Zeiss #10- AF488, Cy2, FITC, GFP
- Zeiss #20- AF555, Rhodamine, TRITC
- Zeiss #26- AF647, Cy5
- Triple (Dapi/FITC/Rhodamine)
- Image data management:
- Please always transfer your data after you finish imaging. Any images left on the computer hard-drives may be deleted on a regular basis to restore hard drive space.
- Image analysis workstation in D11-37- Dell tower, dual color monitors, Wacom tablet
Fees and Invoicing
Please visit the Fee page for information on costs and billing.