Our mission is to improve human and animal health by accelerating research using histology and light microscopy procedures.
The Molecular Pathology Core (MPC) has been in continuous operations since January 1, 2001 and is based in the Experimental Pathology Division of the Department of Pathology, Immunology, and Laboratory Medicine , University of Florida.
The MPC is a comprehensive histology and light microscopy facility for paraffin and frozen blocks and all aspects of sample preparation, fixation, embedding, sectioning, staining, and imaging. Consultations, technical assistance and training are also provided. Standard operating procedures compliant with good laboratory and clinical processes are followed for all procedures.
Services:
- Free NBF fixative and cassettes for users
- Embedding and sectioning frozen and fixed samples by cryostat, microtome, or vibratome
- Staining for routine or specialized histology stains and immunolocalization of proteins or RNA
- DNase/RNase free sample cutting
- Whole slide scans (brightfield), brightfield and fluorescence microscopy, tissue microarrays (TMA), laser microdissection microscopy
- Nanostring GeoMX digital spatial profiling for proteins and whole transcriptome analyses
- Nanostring nCounter assays
- Rental equipment: cryostat, microtomes, vibratome, microscopes (brightfield and fluorescent), and image analysis computer workstations
Acknowledgments
Proper citation: University of Florida Molecular Pathology Core, RRID:SCR_016601.
For those using the Leica 7000 LMD microscope, please include the following: “This work utilized a LEICA 7000 laser microdissection microscope purchased with an NIH shared instrumentation grant 1S10OD016350-01 (RRID:SCR_016601)”.
Please acknowledge services in your manuscripts using the proper citation and co-authorship for staff member who made significant contributions.