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Molecular Pathology Core

MPC Staff Contribute to Biomaterials Advances Publication on Aptamer-Functionalized Hydrogels

We are pleased to highlight a recent publication by Dr. Nikolaos Pipis in Biomaterials Advances (Elsevier), titled “Injectable aptamer-functionalized nucleic acid–collagen hydrogels as a bioactive platform for angiogenic applications.” This work represents the culmination of Dr. Pipis’s doctoral research at the University of Florida and presents the first in vivo…

Olympus VS200 Brightfield&Fluorescence Scanner Installed November 2023

The Olympus VS200 slide scanner was obtained through an NIH Shared Instrumentation Grant and has been installed in D11-35.  Assisted scanning will be performed by MPC staff and charged on a per-slide cost basis. Unassisted use can be performed following training. The VS200 produces fast scans at 20x or 40x…

GeoMX Digital Spatial Profiling

The Nanostring GeoMX Digital Spatial Profiling (DSP) and nCounter instruments are available through the Molecular Pathology Core. The GeoMX provides digital spatial profiling for proteins or mRNA for human and mouse samples. More information about GeoMX DSP can be found at their website. Cell types of interest can be identified…

nCounter Prep Station and Digital Analyzer 5s

The Nanostring nCounter digital analyzer is available through the Molecular Pathology Core and provides robust information about proteins or genes for human, mouse, and rat samples. More details about nCounter assays can be found on their website. Dr. Ann Fu provides technical support for running the instrument for users…

Quadruple IHC Stain

Quadruple or multiplex IHC is becoming more mainstream. Shown below are islets from three species (human, rat, and pig) stained for somatostatin (SS, green), glucagon (GCG, red), insulin (Ins, blue) and pancreatic polypeptide (PP, black). This staining was done using OPAL chromogen kits given to the MPC by Biocare Medical.